Interaction Scheme

Molecule

L his
L-His

Host

Cb7
CB7

c = 10.0 µM

Indicator

Untitled
Dapoxyl

c = 2.5 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 400.0 ± 40.0 M-1
Kd =
logKa = 2.6 ± 0.04
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -14.85 ± 0.25 -3.55 ± 0.06
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 336.0 nm
𝛌em = 380.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM ammonium acetate pH-6.0
Solvents water 100.0 %
Additives Ammonium acetate 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 6.0
Please find here information about the dataset this interaction is part of.
Citation:

W. M. Nau, V. D. Uzunova, A. Hennig, D. M. Bailey, SupraBank 2024, Supramolecular Tandem Enzyme Assays for Multiparameter Sensor Arrays and Enantiomeric Excess Determination of Amino Acids (dataset). https://doi.org/10.34804/supra.20210928260

Link: https://doi.org/10.34804/supra.20210928260
Export: BibTex | RIS | EndNote
Please find here information about the scholarly article describing the results derived from that data.
Citation:

D. M. Bailey, A. Hennig, V. D. Uzunova, W. M. Nau, Chem. Eur. J. 2008, 14, 6069–6077.

Link: https://doi.org/10.1002/chem.200800463
Export: BibTex | RIS | EndNote

Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of L-His (0.05 M) and CB7 (0 — 0.1 M).