Dataset curated
- DOI:
- https://doi.org/10.34804/supra.20220628445
- Title:
- Fluorescent artificial receptor-based membrane assay (FARMA) for spatiotemporally resolved monitoring of biomembrane permeability
- Description:
- AbstractThe spatiotemporally resolved monitoring of membrane translocation, e.g., of drugs or toxins, has been a long-standing goal. Herein, we introduce the fluorescent artificial receptor-based membrane assay (FARMA), a facile, label-free method. With FARMA, the permeation of more than hundred organic compounds (drugs, toxins, pesticides, neurotransmitters, peptides, etc.) through vesicular phospholipid bilayer membranes has been monitored in real time (µs-h time scale) and with high sensitivity (nM-µM concentration), affording permeability coefficients across an exceptionally large range from 10–9–10–3 cm s–1. From a fundamental point of view, FARMA constitutes a powerful tool to assess structure-permeability relationships and to test biophysical models for membrane passage. From an applied perspective, FARMA can be extended to high-throughput screening by adaption of the microplate reader format, to spatial monitoring of membrane permeation by microscopy imaging, and to the compartmentalized monitoring of enzymatic activity.
| Citation: |
W. M. Nau, F. Biedermann, A. Hennig, G. Ghale, SupraBank 2025, Fluorescent artificial receptor-based membrane assay (FARMA) for spatiotemporally resolved monitoring of biomembrane permeability (dataset). https://doi.org/10.34804/supra.20220628445 |
| Link: | https://doi.org/10.34804/supra.20220628445 |
| Export: | BibTex | RIS | EndNote |
| Views | Downloads | Citations |
|---|---|---|
| 17384 | 0 | 0 |
| Citation: |
F. Biedermann, G. Ghale, A. Hennig, W. M. Nau, Commun Biol 2020, 3, DOI 10.1038/s42003-020-1108-9. |
| Link: | https://doi.org/10.1038/s42003-020-1108-9 |
| Export: | BibTex | RIS | EndNote | |
- Creators:
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Frank Biedermann
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Garima Ghale
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Andreas Hennig
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Werner M. Nau
- Contributers:
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Frank Biedermann
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DataManager
- Subjects:
- general biochemistry genetics and molecular biology general agricultural and biological sciences medicine (miscellaneous)
Included Interactions 43 Info
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