Interaction Scheme

Molecule

Coptisine
Coptisine

c = 1.0 µM

Host

Cb6
CB6

c = 0.0 — 375.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.28⋅104 ± M-1
Kd =
logKa = 4.11 ± 0.0
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -23.44 ± 0.0 -5.6 ± 0.0
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 357.0 nm
𝛌em = 523.0 nm
IboundIfree = 3.0
Detailed information about the solvation.
Solvent System Buffer System 120 mM Britton–Robinson pH-2.0
Solvents water
Additives acetic acid 40.0 mM
Phosphoric acid 40.0 mM
BORIC ACID 40.0 mM
Sodium hydroxide
Source of Concentration real
Total concentration 120.0 mM
pH 2.0
Please find here information about the dataset this interaction is part of.
Citation:

C. Li, L. Du, W. Wu, A. Sheng, SupraBank 2024, Supramolecular interaction of cucurbit[n]urils and coptisine by spectrofluorimetry and its analytical application (dataset). https://doi.org/10.34804/supra.20210928313

Link: https://doi.org/10.34804/supra.20210928313
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

C.-F. Li, L.-M. Du, W.-Y. Wu, A.-Z. Sheng, Talanta 2010, 80, 1939–1944.

Link: https://doi.org/10.1016/j.talanta.2009.10.049
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Coptisine (0.0015625 M) and CB6 (0 — 0.003125 M).