Interaction Scheme

Molecule

Untitled
Histidinol

c = 0.0 — 500.0 µM

Host

Napthalene monofunctionalized cb6
Napthalene-monofunctionalized CB6

c = 3.0 µM

Indicator

Europium trinitrate hexahydrate
Europium trinitrate hexahydrate

c = 300.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2900.0 ± 580.0 M-1
Kd =
logKa = 3.46 ± 0.09
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -19.76 ± 0.5 -4.72 ± 0.12
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 266.0 nm
𝛌em = 319.0 nm
Detailed information about the solvation.
Solvent System Single Solvent
Solvent water
pH 7.0
Please find here information about the dataset this interaction is part of.
Citation:

T. Minami, N. A. Esipenko, B. Zhang, L. Isaacs, P. Anzenbacher, SupraBank 2024, “Turn-on” fluorescent sensor array for basic amino acids in water (dataset). https://doi.org/10.34804/supra.202109281

Link: https://doi.org/10.34804/supra.202109281
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

T. Minami, N. A. Esipenko, B. Zhang, L. Isaacs, P. Anzenbacher, Chem. Commun. 2014, 50, 61–63.

Link: https://doi.org/10.1039/c3cc47416j
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Histidinol (0.006896551724137931 M) and Napthalene-monofunctionalized CB6 (0 — 0.013793103448275862 M).