Interaction Scheme

Molecule

Untitled
Lidocaine hydrochloride

c = 0.0 — 2400.0 µM

Host

Acylic 2 2' %281 4 phenylenebis%28oxy%29%29diacetic acid  cucurbituril %28tetrameric glycouril unit%29
CB5a

c = 10.55 µM

Indicator

Untitled
Rhodamine 6G

c = 11.62 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2.60⋅104 ± 1000.0 M-1
Kd =
logKa = 4.41 ± 0.02
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -25.2 ± 0.1 -6.02 ± 0.02
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Absorbance
Detailed information about the solvation.
Solvent System Buffer System 25 mM sodium phosphate buffer, pH 7.4
Source of Concentration
Total concentration 25.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

L. Isaacs, P. Y. Zavalij, D. Ma, R. Glassenberg, S. Ghosh, SupraBank 2024, Acyclic cucurbituril congener binds to local anaesthetics (dataset). https://doi.org/10.34804/supra.20240423551

Link: https://doi.org/10.34804/supra.20240423551
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

D. Ma, R. Glassenberg, S. Ghosh, P. Y. Zavalij, L. Isaacs, Supramolecular Chemistry 2012, 24, 325–332.

Link: https://doi.org/10.1080/10610278.2012.658394
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Lidocaine hydrochloride (0.0007692307692307692 M) and CB5a (0 — 0.0015384615384615385 M).