Interaction Scheme

Molecule

Untitled
Dapoxyl

Host

Cb7
CB7

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2.00⋅104 ± 2000.0 M-1
Kd =
logKa = 4.3 ± 0.04
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -24.55 ± 0.25 -5.87 ± 0.06
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 336.0 nm
𝛌em = 380.0 nm
IboundIfree = 200.0
Detailed information about the solvation.
Solvent System Buffer System 10 mM ammonium acetate pH-6.0
Solvents water 100.0 %
Additives Ammonium acetate 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 6.0
Please find here information about the dataset this interaction is part of.
Citation:

A. Hennig, H. Bakirci, W. M. Nau, SupraBank 2022, Label-free continuous enzyme assays with macrocycle-fluorescent dye complexes (dataset). https://doi.org/10.34804/supra.20210928224

Link: https://doi.org/10.34804/supra.20210928224
Export: BibTex | RIS | EndNote
Please find here information about the scholarly article describing the results derived from that data.
Citation:

A. Hennig, H. Bakirci, W. M. Nau, Nat Methods 2007, 4, 629–632.

Link: https://doi.org/10.1038/NMETH1064
Export: BibTex | RIS | EndNote |

Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Dapoxyl (0.001 M) and CB7 (0 — 0.002 M).