Interaction

curated

Interaction Scheme

Molecule

Rhodamine 800
Rhodamine 800

c = 0.04 µM

Host

Scx8
sCx8

c = 0.0 — 52.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 4.55⋅105 M-1
Kd =
logKa = 5.66
T 25.0 °C ≈ 298 K
kin= 0.0 ± M-1s-1
kout= ± s-1
Energy kJ mol-1 kcal mol-1
ΔG = -32.29 -7.72
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 600.0 nm
𝛌em = 710.0 nm
Ibound⁄Ifree = 3.3
Detailed information about the solvation.
Solvent System Buffer System PBS pH-7.2
Solvents water 100.0 %
Source of Concentration
pH 7.2
Please find here information about the dataset this interaction is part of.
Citation:

T. Jin, SupraBank 2025, Near-Infrared Fluorescence Detection of Acetylcholine in Aqueous Solution Using a Complex of Rhodamine 800 and p-Sulfonato-calix[8]arene (dataset). https://doi.org/10.34804/supra.20210928378

Link: https://doi.org/10.34804/supra.20210928378
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

T. Jin, Sensors 2010, 10, 2438–2449.

Link: https://doi.org/10.3390/s100302438
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Rhodamine 800 (4.4000044000044e-05 M) and sCx8 (0 — 8.8000088000088e-05 M).