Interaction Scheme
Molecule
N-methyl-N-(4-(2-(1-methylpyridin-4(1H)-ylidene...
c = 50.0 µM
Host
sCx6
c = 0.0 — 1000.0 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 1.00⋅105 | M-1 | |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -28.54 | -6.82 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 470.0 nm | ||
| 𝛌em | = | 570.0 nm | ||
| Ibound⁄Ifree | = | 0.02 | ||
Detailed information about the solvation.
| Solvent System | Buffer System | phosphate MeOH pH-7.2 |
| Solvents | water | 95.0 % |
| methanol | 5.0 % | |
| Additives | Sodium hydroxide | |
| Sodium dihydro... | ||
| Source of Concentration | estimated | |
| pH | 7.2 |
Please find here information about the dataset this interaction is part of.
| Citation: |
N. Korbakov, P. Timmerman, N. Lidich, B. Urbach, A. Sa'ar, S. Yitzchaik, SupraBank 2025, Acetylcholine Detection at Micromolar Concentrations with the Use of an Artificial Receptor-Based Fluorescence Switch (dataset). https://doi.org/10.34804/supra.20210928358 |
| Link: | https://doi.org/10.34804/supra.20210928358 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
N. Korbakov, P. Timmerman, N. Lidich, B. Urbach, A. Sa'ar, S. Yitzchaik, Langmuir 2008, 24, 2580–2587. |
| Link: | https://doi.org/10.1021/la703010z |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of N-methyl-N-(4-(2-(1-methylpyridin-4(1H)-ylidene)ethylidene)cyclohexa-2,5-dien-1-ylidene)methanaminium (0.0002 M) and sCx6 (0 — 0.0004 M).
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