Interaction

curated

Interaction Scheme

Molecule

Histamine
Histamine

Host

Cb7
CB7

c = 10.0 µM

Indicator

Untitled
Dapoxyl

c = 2.5 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 3.20⋅104 ± 3200.0 M-1
Kd =
logKa = 4.51 ± 0.04
T 30.0 °C ≈ 303 K
Energy kJ mol-1 kcal mol-1
ΔG = -26.15 ± 0.25 -6.25 ± 0.06
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 336.0 nm
𝛌em = 380.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM phosphate pH-7.4
Solvents water 100.0 %
Additives Disodium phosph... 7.5 mM
Sodium dihydro... 2.5 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

W. M. Nau, V. D. Uzunova, A. Hennig, D. M. Bailey, SupraBank 2024, Supramolecular Tandem Enzyme Assays for Multiparameter Sensor Arrays and Enantiomeric Excess Determination of Amino Acids (dataset). https://doi.org/10.34804/supra.20210928260

Link: https://doi.org/10.34804/supra.20210928260
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

D. M. Bailey, A. Hennig, V. D. Uzunova, W. M. Nau, Chem. Eur. J. 2008, 14, 6069–6077.

Link: https://doi.org/10.1002/chem.200800463
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Histamine (0.000625 M) and CB7 (0 — 0.00125 M).