Interaction Scheme
Molecule
putrescine (fully protonated form)
Host
CB7
c = 30.0 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 3.00⋅105 | ± 1.00⋅104 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 30.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -31.79 | ± 0.08 | -7.6 | ± 0.02 |
| ΔH | = | -13.8 | ± 0.41 | -3.3 | ± 0.1 |
| -TΔS | = | -18.0 | ± 0.41 | -4.3 | ± 0.1 |
| J mol-1 K-1 | cal mol-1 K-1 | ||||
| ΔS | = | 59.4 | ± 1.4 | 14.2 | ± 0.3 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | ||
| Assay Type: | Direct Binding Assay | ||
| Technique: | Isothermal Titration Calorimetry | ||
| Molecule: | syringe | ||
| Partner: | cell | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM ammonium acetate pH-6.0 |
| Solvents | water | 100.0 % |
| Additives | Ammonium acetate | 10.0 mM |
| Source of Concentration | ||
| Total concentration | 10.0 mM | |
| pH | 6.0 |
Please find here information about the dataset this interaction is part of.
| Citation: |
W. M. Nau, V. D. Uzunova, A. Hennig, D. M. Bailey, SupraBank 2024, Supramolecular Tandem Enzyme Assays for Multiparameter Sensor Arrays and Enantiomeric Excess Determination of Amino Acids (dataset). https://doi.org/10.34804/supra.20210928260 |
| Link: | https://doi.org/10.34804/supra.20210928260 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
D. M. Bailey, A. Hennig, V. D. Uzunova, W. M. Nau, Chem. Eur. J. 2008, 14, 6069–6077. |
| Link: | https://doi.org/10.1002/chem.200800463 |
| Export: | BibTex | RIS | EndNote |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of putrescine (fully protonated form) (6.666666666666667e-05 M) and CB7 (0 — 0.00013333333333333334 M).
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