Interaction

curated

Interaction Scheme

Molecule

Ao hcl
AO HCl

c = 2.2 µM

Host

Beta cd
β-CD

c = 0.0 — 2.5 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1200.0 ± 300.0 M-1
Kd =
logKa = 3.08 ± 0.11
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -17.58 ± 0.63 -4.2 ± 0.15
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 429.0 nm
𝛌em = 548.0 nm
Ibound⁄Ifree = 0.75
Detailed information about the solvation.
Solvent System Complex Mixture
Solvents water
Additives Sodium hydroxide
pH 12.0
Please find here information about the dataset this interaction is part of.
Citation:

J. Mohanty, H. Pal, W. M. Nau, M. Shaikh, P. K. Singh, SupraBank 2024, Complexation of acridine orange by cucurbit[7]uril and β-cyclodextrin: photophysical effects and pKashifts (dataset). https://doi.org/10.34804/supra.20210928392

Link: https://doi.org/10.34804/supra.20210928392
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

M. Shaikh, J. Mohanty, P. K. Singh, W. M. Nau, H. Pal, Photochem. Photobiol. Sci. 2008, 7, 408–414.

Link: https://doi.org/10.1039/B715815G
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of AO HCl (0.016666666666666666 M) and β-CD (0 — 0.03333333333333333 M).