Interaction

curated

Interaction Scheme

Molecule

Untitled
PLP

c = 0.0 — 26.51 µM

Host

Gc5a
gCx5-6C

c = 0.8 µM

Indicator

Uranine
Uranine

c = 1.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2.00⋅106 ± 5.00⋅105 M-1
Kd =
logKa = 6.3 ± 0.11
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -35.97 ± 0.63 -8.6 ± 0.15
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
Detailed information about the solvation.
Solvent System Buffer System 10 mM HEPES 2mM MgCl2 pH-7.4
Solvents water 100.0 %
Additives Hepes 10.0 mM
MAGNESIUM CHLORIDE 2.0 mM
Source of Concentration
Total concentration 12.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

Y. Yue, X. Hu, Z. Zheng, Y. Kong, F. Yang, D. Guo, SupraBank 2024, Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein (dataset). https://doi.org/10.34804/supra.20210928191

Link: https://doi.org/10.34804/supra.20210928191
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

Y. X. Yue, Y. Kong, F. Yang, Z. Zheng, X. Y. Hu, D. S. Guo, ChemistryOpen 2019, 8, 1437–1440.

Link: https://doi.org/10.1002/open.201900316
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of PLP (1.0e-05 M) and gCx5-6C (0 — 2.0e-05 M).