Interaction Scheme
Molecule
ARTK(me3)QTARKSTGY
Host
sCx4-NHSO2(4-CO2H)Ph
Indicator
Lucigenin
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 6.17⋅107 | ± 2.28⋅106 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -44.47 | ± 0.09 | -10.63 | ± 0.02 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Competitive | |||
| Assay Type: | Competitive Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 369.0 nm | ||
| 𝛌em | = | 485.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM phosphate pH-7.4 |
| Solvents | water | 100.0 % |
| Additives | Disodium phosph... | 7.5 mM |
| Sodium dihydro... | 2.5 mM | |
| Source of Concentration | ||
| Total concentration | 10.0 mM | |
| pH | 7.4 |
Please find here information about the dataset this interaction is part of.
| Citation: |
M. Ali, K. D. Daze, D. E. Strongin, S. B. Rothbart, H. Rincon-Arano, H. F. Allen, J. Li, B. D. Strahl, F. Hof, T. G. Kutateladze, SupraBank 2024, Molecular Insights into Inhibition of the Methylated Histone-Plant Homeodomain Complexes by Calixarenes (dataset). https://doi.org/10.34804/supra.202109282 |
| Link: | https://doi.org/10.34804/supra.202109282 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
M. Ali, K. D. Daze, D. E. Strongin, S. B. Rothbart, H. Rincon-Arano, H. F. Allen, J. Li, B. D. Strahl, F. Hof, T. G. Kutateladze, Journal of Biological Chemistry 2015, 290, 22919–22930. |
| Link: | https://doi.org/10.1074/jbc.M115.669333 |
| Export: | BibTex | RIS | EndNote |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of ARTK(me3)QTARKSTGY (3.241491085899514e-07 M) and sCx4-NHSO2(4-CO2H)Ph (0 — 6.482982171799028e-07 M).
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