Interaction Scheme
Molecule
Lucigenin
c = 1.0 µM
Host
sCx5
c = 0.0 — 9.15 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 1.48⋅106 | ± 2.10⋅105 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -35.22 | ± 0.35 | -8.42 | ± 0.08 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 368.0 nm | ||
| 𝛌em | = | 505.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM HEPES pH-7.4 |
| Solvents | water | 100.0 % |
| Additives | Hepes | 10.0 mM |
| Source of Concentration | ||
| Total concentration | 10.0 mM | |
| pH | 7.4 |
Please find here information about the dataset this interaction is part of.
| Citation: |
D. Guo, J. Gao, Z. Zheng, W. Geng, H. Yu, Y. Wang, SupraBank 2024, Facile Fluorescence Monitoring of Gut Microbial Metabolite Trimethylamine N-oxide via Molecular Recognition of Guanidinium-Modified Calixarene (dataset). https://doi.org/10.34804/supra.20210928243 |
| Link: | https://doi.org/10.34804/supra.20210928243 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
H. Yu, W.-C. Geng, Z. Zheng, J. Gao, D.-S. Guo, Y. Wang, Theranostics 2019, 9, 4624–4632. |
| Link: | https://doi.org/10.7150/thno.33459 |
| Export: | BibTex | RIS | EndNote |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of Lucigenin (1.3513513513513513e-05 M) and sCx5 (0 — 2.7027027027027027e-05 M).
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