Interaction Scheme
Molecule
Rhodamine 123
c = 1.0 µM
Host
Cx4-(N2-Ph-COOH)
c = 0.0 — 8.1 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 1.40⋅107 | ± 1.00⋅106 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -40.79 | ± 0.18 | -9.75 | ± 0.04 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 500.0 nm | ||
| 𝛌em | = | 525.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 149.7 mM PBS pH-7.4 |
| Solvents | water | |
| Additives | sodium chloride | 137.0 mM |
| Disodium hydrog... | 6.1 mM | |
| Sodium dihydrog... | 3.9 mM | |
| potassium chloride | 2.7 mM | |
| Sodium hydroxide | ||
| Source of Concentration | ||
| Total concentration | 149.7 mM | |
| pH | 7.4 |
Please find here information about the dataset this interaction is part of.
| Citation: |
D. Guo, Z. Zheng, Z. Li, W. Geng, S. Jia, D. Ding, SupraBank 2025, A Noncovalent Fluorescence Turn‐on Strategy for Hypoxia Imaging (dataset). https://doi.org/10.34804/supra.20210928222 |
| Link: | https://doi.org/10.34804/supra.20210928222 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
W. C. Geng, S. Jia, Z. Zheng, Z. Li, D. Ding, D. S. Guo, Angew. Chem. Int. Ed. 2019, 58, 2377–2381. |
| Link: | https://doi.org/10.1002/anie.201813397 |
| Export: | BibTex | RIS | EndNote |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of Rhodamine 123 (1.4285714285714286e-06 M) and Cx4-(N2-Ph-COOH) (0 — 2.8571428571428573e-06 M).
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