Interaction

curated

Interaction Scheme

Molecule

Lysine
D/L-Lys

c = 0.0 — 8.0 µM

Host

Cb7
CB7

c = 10.0 µM

Indicator

Untitled
Dapoxyl

c = 2.5 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 870.0 ± 87.0 M-1
Kd =
logKa = 2.94 ± 0.04
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -16.78 ± 0.25 -4.01 ± 0.06
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 336.0 nm
𝛌em = 380.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM ammonium acetate pH-6.0
Solvents water 100.0 %
Additives Ammonium acetate 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 6.0
Please find here information about the dataset this interaction is part of.
Citation:

A. Hennig, H. Bakirci, W. M. Nau, SupraBank 2024, Label-free continuous enzyme assays with macrocycle-fluorescent dye complexes (dataset). https://doi.org/10.34804/supra.20210928224

Link: https://doi.org/10.34804/supra.20210928224
Export: BibTex | RIS | EndNote
Please find here information about the scholarly article describing the results derived from that data.
Citation:

A. Hennig, H. Bakirci, W. M. Nau, Nat Methods 2007, 4, 629–632.

Link: https://doi.org/10.1038/NMETH1064
Export: BibTex | RIS | EndNote |

Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of D/L-Lys (0.022988505747126436 M) and CB7 (0 — 0.04597701149425287 M).