Interaction

curated

Interaction Scheme

Molecule

Lysine
D/L-Lys

c = 0.0 — 200.0 µM

Host

Cx4
Cx4

c = 100.0 µM

Indicator

Untitled
2,3-Diazabicyclo(2.2.2)oct-2-ene

c = 100.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1000.0 ± 100.0 M-1
Kd =
logKa = 3.0 ± 0.04
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -17.12 ± 0.25 -4.09 ± 0.06
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 365.0 nm
𝛌em = 450.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM ammonium acetate pH-6.0
Solvents water 100.0 %
Additives Ammonium acetate 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 6.0
Please find here information about the dataset this interaction is part of.
Citation:

A. Hennig, H. Bakirci, W. M. Nau, SupraBank 2024, Label-free continuous enzyme assays with macrocycle-fluorescent dye complexes (dataset). https://doi.org/10.34804/supra.20210928224

Link: https://doi.org/10.34804/supra.20210928224
Export: BibTex | RIS | EndNote
Please find here information about the scholarly article describing the results derived from that data.
Citation:

A. Hennig, H. Bakirci, W. M. Nau, Nat Methods 2007, 4, 629–632.

Link: https://doi.org/10.1038/NMETH1064
Export: BibTex | RIS | EndNote |

Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of D/L-Lys (0.02 M) and Cx4 (0 — 0.04 M).