Interaction

curated

Interaction Scheme

Molecule

Histamine
Histamine

c = 0.0 — 200.0 µM

Host

Cx4
Cx4

c = 100.0 µM

Indicator

Untitled
2,3-Diazabicyclo(2.2.2)oct-2-ene

c = 100.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 9.00⋅104 ± 9000.0 M-1
Kd =
logKa = 4.95 ± 0.04
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -28.28 ± 0.25 -6.76 ± 0.06
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 365.0 nm
𝛌em = 450.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM ammonium acetate pH-6.0
Solvents water 100.0 %
Additives Ammonium acetate 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 6.0
Please find here information about the dataset this interaction is part of.
Citation:

A. Hennig, H. Bakirci, W. M. Nau, SupraBank 2024, Label-free continuous enzyme assays with macrocycle-fluorescent dye complexes (dataset). https://doi.org/10.34804/supra.20210928224

Link: https://doi.org/10.34804/supra.20210928224
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

A. Hennig, H. Bakirci, W. M. Nau, Nat Methods 2007, 4, 629–632.

Link: https://doi.org/10.1038/NMETH1064
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Histamine (0.00022222222222222223 M) and Cx4 (0 — 0.00044444444444444447 M).