Interaction Scheme
Molecule
WGGGQTARK(me3)STGGR
c = 900.0 — 1200.0 µM
Host
sCx4-TEG-NBD
c = 81.0 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 2.10⋅105 | ± 6.50⋅104 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -30.38 | ± 0.79 | -7.26 | ± 0.19 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | ||
| Assay Type: | Direct Binding Assay | ||
| Technique: | Isothermal Titration Calorimetry | ||
| Molecule: | syringe | ||
| Partner: | syringe | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM potassium phosphate pH-7.4 |
| Solvents | water | |
| Additives | Dipotassium hyd... | 7.0 mM |
| Potassium dihyd... | 3.0 mM | |
| Source of Concentration | ||
| Total concentration | 10.0 mM | |
| pH | 7.4 |
Please find here information about the dataset this interaction is part of.
| Citation: |
I. N. Gober, M. L. Waters, SupraBank 2025, Supramolecular Affinity Labeling of Histone Peptides Containing Trimethyllysine and Its Application to Histone Deacetylase Assays (dataset). https://doi.org/10.34804/supra.20210928161 |
| Link: | https://doi.org/10.34804/supra.20210928161 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
I. N. Gober, M. L. Waters, J. Am. Chem. Soc. 2016, 138, 9452–9459. |
| Link: | https://doi.org/10.1021/jacs.6b02836 |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of WGGGQTARK(me3)STGGR (9.523809523809524e-05 M) and sCx4-TEG-NBD (0 — 0.00019047619047619048 M).
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