Interaction Scheme


Rhodamine b
Rhodamine B

c = 4.08 µM



c = 400.0 — 1140.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 5050.0 ± 40.0 M-1
Kd =
logKa = 3.7 ± 0.0
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -21.14 ± 0.02 -5.05 ± 0.0
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
Detailed information about the solvation.
Solvent System Buffer System 100 mM citrate pH-6.0
Solvents water
Additives Trisodium citra... 82.5 mM
citric acid 17.5 mM
Source of Concentration real
Total concentration 100.0 mM
pH 6.0
Please find here information about the dataset this interaction is part of.

D. Guo, Y. Liu, C. Li, Z. Pan, Z. Li, SupraBank 2022, A Comparative Study of Complexation of β-Cyclodextrin, Calix[4]arenesulfonate and Cucurbit[7]uril with Dye Guests: Fluorescence Behavior and Binding Ability (dataset).

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Please find here information about the scholarly article describing the results derived from that data.

Y. Liu, C.-J. Li, D.-S. Guo, Z.-H. Pan, Z. Li, Supramolecular Chemistry 2007, 19, 517–523.

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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Rhodamine B (0.0039603960396039604 M) and CB7 (0 — 0.007920792079207921 M).