Interaction Scheme

Molecule

H leu arg arg trp pser leu gly oh
H-Leu-Arg-Arg-Trp-pSer-Leu-Gly-OH

Host

Scx4 5c
sCx4-5C

Indicator

Lucigenin
Lucigenin

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.50⋅104 M-1
Kd =
logKa = 4.18
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -23.84 -5.7
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 367.0 nm
𝛌em = 506.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM HEPES pH-7.0
Solvents water
Additives Hepes 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.0
Please find here information about the dataset this interaction is part of.
Citation:

D. Guo, W. Nau, Y. Pan, A. Barba-Bon, A. Hennig, S. Peng, SupraBank 2024, Phosphorylation-Responsive Membrane Transport of Peptides (dataset). https://doi.org/10.34804/supra.20210928233

Link: https://doi.org/10.34804/supra.20210928233
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

S. Peng, A. Barba-Bon, Y.-C. Pan, W. M. Nau, D.-S. Guo, A. Hennig, Angew. Chem. Int. Ed. 2017, 56, 15742–15745.

Link: https://doi.org/10.1002/anie.201707979
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of H-Leu-Arg-Arg-Trp-pSer-Leu-Gly-OH (0.0013333333333333333 M) and sCx4-5C (0 — 0.0026666666666666666 M).