Interaction Scheme
Molecule
H-Trp-Lys-Arg-pThr-Leu-Arg-Arg-Leu-OH
Host
sCx4-5C
c = 3.5 µM
Indicator
Lucigenin
c = 0.5 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 1.20⋅105 | M-1 | |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -28.99 | -6.93 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Competitive | |||
| Assay Type: | Competitive Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 367.0 nm | ||
| 𝛌em | = | 506.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM HEPES pH-7.5 |
| Solvents | water | |
| Additives | Hepes | 10.0 mM |
| Source of Concentration | ||
| Total concentration | 10.0 mM | |
| pH | 7.5 |
Please find here information about the dataset this interaction is part of.
| Citation: |
D. Guo, W. Nau, Y. Pan, A. Barba-Bon, A. Hennig, S. Peng, SupraBank 2024, Phosphorylation-Responsive Membrane Transport of Peptides (dataset). https://doi.org/10.34804/supra.20210928233 |
| Link: | https://doi.org/10.34804/supra.20210928233 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
S. Peng, A. Barba-Bon, Y.-C. Pan, W. M. Nau, D.-S. Guo, A. Hennig, Angew. Chem. Int. Ed. 2017, 56, 15742–15745. |
| Link: | https://doi.org/10.1002/anie.201707979 |
| Export: | BibTex | RIS | EndNote |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of H-Trp-Lys-Arg-pThr-Leu-Arg-Arg-Leu-OH (0.00016666666666666666 M) and sCx4-5C (0 — 0.0003333333333333333 M).
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