Interaction

curated

Interaction Scheme

Molecule

Untitled
RB

c = 0.0 — 1.6 µM

Host

Gc5a
gCx5-6C

c = 0.6 µM

Indicator

Fluorescein
Fluorescein

c = 0.5 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 9.60⋅107 ± 2.00⋅106 M-1
Kd =
logKa = 7.98 ± 0.01
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -45.56 ± 0.05 -10.89 ± 0.01
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 500.0 nm
𝛌em = 512.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM HEPES pH-7.4
Solvents water 100.0 %
Additives Hepes 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

J. Gao, F. Chen, D. Guo, Z. Zheng, W. Geng, J. Li, X. Duan, D. Ding, SupraBank 2024, Biomarker Displacement Activation: A General Host–Guest Strategy for Targeted Phototheranostics in Vivo (dataset). https://doi.org/10.34804/supra.20210928231

Link: https://doi.org/10.34804/supra.20210928231
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

J. Gao, J. Li, W.-C. Geng, F.-Y. Chen, X. Duan, Z. Zheng, D. Ding, D.-S. Guo, J. Am. Chem. Soc. 2018, 140, 4945–4953.

Link: https://doi.org/10.1021/jacs.8b02331
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of RB (2.0833333333333333e-07 M) and gCx5-6C (0 — 4.1666666666666667e-07 M).