Interaction Scheme
Molecule
Adenosine triphosphate
c = 0.0 — 1.3 µM
Host
gCx5-6C
c = 0.4 µM
Indicator
Fluorescein
c = 0.5 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 4.70⋅108 | ± 1.40⋅108 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -49.5 | ± 0.76 | -11.83 | ± 0.18 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Competitive | |||
| Assay Type: | Competitive Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 500.0 nm | ||
| 𝛌em | = | 513.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM HEPES pH-7.4 |
| Solvents | water | 100.0 % |
| Additives | Hepes | 10.0 mM |
| Source of Concentration | ||
| Total concentration | 10.0 mM | |
| pH | 7.4 |
Please find here information about the dataset this interaction is part of.
| Citation: |
J. Gao, D. Guo, Y. Wang, Z. Zheng, H. Sun, W. Geng, SupraBank 2024, Ultrasensitive and specific fluorescence detection of a cancer biomarkerviananomolar binding to a guanidinium-modified calixarene (dataset). https://doi.org/10.34804/supra.20210928227 |
| Link: | https://doi.org/10.34804/supra.20210928227 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
Z. Zheng, W.-C. Geng, J. Gao, Y.-Y. Wang, H. Sun, D.-S. Guo, Chem. Sci. 2018, 9, 2087–2091. |
| Link: | https://doi.org/10.1039/C7SC04989G |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of Adenosine triphosphate (4.25531914893617e-08 M) and gCx5-6C (0 — 8.51063829787234e-08 M).
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