Interaction

curated

Interaction Scheme

Molecule

Fluorescein
Fluorescein

c = 1.0 µM

Host

Gc5a
gCx5-6C

c = 0.0 — 3.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 5.00⋅106 ± 1.00⋅106 M-1
Kd =
logKa = 6.7 ± 0.09
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -38.24 ± 0.5 -9.14 ± 0.12
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 500.0 nm
𝛌em = 513.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM HEPES pH-7.4
Solvents water 100.0 %
Additives Hepes 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

J. Gao, D. Guo, Y. Wang, Z. Zheng, H. Sun, W. Geng, SupraBank 2025, Ultrasensitive and specific fluorescence detection of a cancer biomarkerviananomolar binding to a guanidinium-modified calixarene (dataset). https://doi.org/10.34804/supra.20210928227

Link: https://doi.org/10.34804/supra.20210928227
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

Z. Zheng, W.-C. Geng, J. Gao, Y.-Y. Wang, H. Sun, D.-S. Guo, Chem. Sci. 2018, 9, 2087–2091.

Link: https://doi.org/10.1039/C7SC04989G
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Fluorescein (4.0e-06 M) and gCx5-6C (0 — 8.0e-06 M).