Interaction

curated

Interaction Scheme

Molecule

Safranine t
Safranine T

c = 42.0 µM

Host

Beta cd
β-CD

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 160.0 ± M-1
Kd =
logKa = 2.2 ± 0.0
T 20.0 °C ≈ 293 K
Energy kJ mol-1 kcal mol-1
ΔG = -12.37 ± 0.0 -2.96 ± 0.0
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 525.0 nm
𝛌em = 573.0 nm
Ibound⁄Ifree = 2.5
Detailed information about the solvation.
Solvent System Buffer System 200 mM tris pH-7.2
Solvents water
Additives Trometamol 200.0 mM
hydrochloric acid
Source of Concentration
Total concentration 200.0 mM
pH 7.2
Please find here information about the dataset this interaction is part of.
Citation:

G. Zhang, S. Shuang, C. Dong, Y. Pang, M. M. Choi, D. Liu, SupraBank 2024, Spectroscopic studies on the interaction of Safranine T with DNA in β-cyclodextrin and carboxymethyl-β-cyclodextrin (dataset). https://doi.org/10.34804/supra.20210928338

Link: https://doi.org/10.34804/supra.20210928338
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

G. Zhang, Y. Pang, S. Shuang, C. Dong, M. M. F. Choi, D. Liu, Journal of Photochemistry and Photobiology A: Chemistry 2005, 169, 153–158.

Link: https://doi.org/10.1016/j.jphotochem.2004.06.016
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Safranine T (0.125 M) and β-CD (0 — 0.25 M).