Interaction Scheme

Molecule

Hoechst dye 33258
Hoechst Dye 33258

c = 1.0 µM

Host

Cb7
CB7

c = 0.0 — 125.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.45⋅109 M-1
Kd =
logKa = 9.16
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -52.29 -12.5
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 365.0 nm
𝛌em = 480.0 nm
Detailed information about the solvation.
Solvent System Single Solvent
Solvent water
pH 7.0
Please find here information about the dataset this interaction is part of.
Citation:

J. Mohanty, N. Barooah, H. Pal, A. C. Bhasikuttan, SupraBank 2022, Supramolecular assembly of hoechst-33258 with cucurbit[7]uril macrocycle (dataset). https://doi.org/10.34804/supra.2021092892

Link: https://doi.org/10.34804/supra.2021092892
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

N. Barooah, J. Mohanty, H. Pal, A. C. Bhasikuttan, Phys. Chem. Chem. Phys. 2011, 13, 13117.

Link: https://doi.org/10.1039/c1cp20493a
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Hoechst Dye 33258 (1.3793103448275862e-08 M) and CB7 (0 — 2.7586206896551723e-08 M).