Interaction Scheme
Molecule
Hoechst Dye 33258
c = 1.0 µM
Host
CB7
c = 0.0 — 50.0 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 6.30⋅1011 | M-1 | |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -67.35 | -16.1 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 353.0 nm | ||
| 𝛌em | = | 500.0 nm | ||
Detailed information about the solvation.
| Solvent System | Complex Mixture |
| Solvents | water |
| Additives | hydrochloric acid |
| pH | 4.5 |
Please find here information about the dataset this interaction is part of.
| Citation: |
J. Mohanty, N. Barooah, H. Pal, A. C. Bhasikuttan, SupraBank 2025, Supramolecular assembly of hoechst-33258 with cucurbit[7]uril macrocycle (dataset). https://doi.org/10.34804/supra.2021092892 |
| Link: | https://doi.org/10.34804/supra.2021092892 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
N. Barooah, J. Mohanty, H. Pal, A. C. Bhasikuttan, Phys. Chem. Chem. Phys. 2011, 13, 13117. |
| Link: | https://doi.org/10.1039/c1cp20493a |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of Hoechst Dye 33258 (3.174603174603175e-11 M) and CB7 (0 — 6.34920634920635e-11 M).
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