Interaction

curated

Interaction Scheme

Molecule

Lysine
D/L-Lys

c = 0.0 — 1000.0 µM

Host

Cx4
Cx4

c = 1.5 µM

Indicator

Lucigenin
Lucigenin

c = 1.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka < 1000.0 M-1
Kd <
logKa < 3.0
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG > -17.12 -4.09
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 369.0 nm
𝛌em = 510.0 nm
Detailed information about the solvation.
Solvent System Buffer System 5 mM glycine pH-10
Solvents water
Additives glycine
Source of Concentration estimated
Total concentration 5.0 mM
pH 10.0
Please find here information about the dataset this interaction is part of.
Citation:

M. J. González-Álvarez, W. M. Nau, M. Florea, S. Kudithipudi, A. Rei, A. Jeltsch, SupraBank 2025, A Fluorescence-Based Supramolecular Tandem Assay for Monitoring Lysine Methyltransferase Activity in Homogeneous Solution (dataset). https://doi.org/10.34804/supra.20210928283

Link: https://doi.org/10.34804/supra.20210928283
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

M. Florea, S. Kudithipudi, A. Rei, M. J. González-Álvarez, A. Jeltsch, W. M. Nau, Chem. Eur. J. 2012, 18, 3521–3528.

Link: https://doi.org/10.1002/chem.201103397
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of D/L-Lys (0.02 M) and Cx4 (0 — 0.04 M).