Interaction

curated

Interaction Scheme

Molecule

Auramine o
Auramine O

Host

Alpha cd
α-CD

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 5000.0 ± M-1
Kd =
logKa = 3.7 ± 0.0
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -21.11 ± 0.0 -5.05 ± 0.0
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 365.0 nm
𝛌em = 500.0 nm
Ibound⁄Ifree = 1.5
Detailed information about the solvation.
Solvent System Buffer System phosphate pH-7.0
Solvents water
Source of Concentration
pH 7.0
Please find here information about the dataset this interaction is part of.
Citation:

A. Mwalupindi, SupraBank 2025, Influence of organized media on the absorption and fluorescence spectra of Auramine-O dye (dataset). https://doi.org/10.34804/supra.20210928354

Link: https://doi.org/10.34804/supra.20210928354
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

A. Mwalupindi, Talanta 1994, 41, 599–609.

Link: https://doi.org/10.1016/0039-9140(93)E0033-A
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Auramine O (0.004 M) and α-CD (0 — 0.008 M).