Interaction Scheme
Molecule
Methylene Blue
c = 10.0 µM
Host
sCx6
c = 0.0 — 12.0 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 6.61⋅105 | ± | M-1 |
| Kd = | |||
| logKa = | |||
| T | 20.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -32.66 | ± 0.0 | -7.81 | ± 0.0 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 640.0 nm | ||
| 𝛌em | = | 698.0 nm | ||
| Ibound⁄Ifree | = | 0.2 | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 120 mM Britton–Robinson pH-7.5 |
| Solvents | water | |
| Additives | BORIC ACID | 40.0 mM |
| acetic acid | 40.0 mM | |
| Phosphoric acid | 40.0 mM | |
| hydrochloric acid | ||
| Source of Concentration | ||
| Total concentration | 120.0 mM | |
| pH | 7.5 |
Please find here information about the dataset this interaction is part of.
| Citation: |
Q. Lu, J. Gu, H. Yu, C. Liu, L. Wang, Y. Zhou, SupraBank 2024, Study on the inclusion interaction of p-sulfonated calix[n]arenes with Vitamin K3 using methylene blue as a spectral probe (dataset). https://doi.org/10.34804/supra.2021092893 |
| Link: | https://doi.org/10.34804/supra.2021092893 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
Q. Lu, J. Gu, H. Yu, C. Liu, L. Wang, Y. Zhou, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2007, 68, 15–20. |
| Link: | https://doi.org/10.1016/j.saa.2006.10.044 |
| Export: | BibTex | RIS | EndNote |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of Methylene Blue (3.0257186081694404e-05 M) and sCx6 (0 — 6.051437216338881e-05 M).
Please sign in: customize the simulation by signing in to the SupraBank.
