Interaction Scheme
Molecule
Recombinant insulin human
Host
CB8
c = 20.0 µM
Cofactor
MDPP
c = 20.0 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 3.30⋅105 | ± 3.30⋅104 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 22.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -31.5 | ± 0.25 | -7.53 | ± 0.06 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Associative Binding Assay | |||
| Technique: | Absorbance | |||
| 𝛌abs | = | 443.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM phosphate pH-2.7 |
| Solvents | water | |
| Source of Concentration | real | |
| Total concentration | 10.0 mM | |
| pH | 2.7 |
Please find here information about the dataset this interaction is part of.
| Citation: |
W. M. Nau, F. Biedermann, SupraBank 2025, Noncovalent Chirality Sensing Ensembles for the Detection and Reaction Monitoring of Amino Acids, Peptides, Proteins, and Aromatic Drugs (dataset). https://doi.org/10.34804/supra.20210928387 |
| Link: | https://doi.org/10.34804/supra.20210928387 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
F. Biedermann, W. M. Nau, Angew. Chem. Int. Ed. 2014, 53, 5694–5699. |
| Link: | https://doi.org/10.1002/anie.201400718 |
| Export: | BibTex | RIS | EndNote |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of Recombinant insulin human (6.0606060606060605e-05 M) and CB8 (0 — 0.00012121212121212121 M).
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