Interaction

curated

Interaction Scheme

Molecule

Oleate
oleate

c = 0.0 — 4.03 µM

Host

Gcx5 12c
Guanidinocalix[5]arene pentadodecyl ether

c = 1.0 µM

Indicator

Fluorescein
Fluorescein

c = 0.5 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 6.10⋅106 ± 8.00⋅105 M-1
Kd =
logKa = 6.79 ± 0.06
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -38.73 ± 0.33 -9.26 ± 0.08
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 500.0 nm
𝛌em = 512.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM HEPES pH-7.4
Solvents water 100.0 %
Additives Hepes 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

Z. Li, D. Guo, W. Geng, D. Zhang, C. Gong, K. M. Barraza, J. Beauchamp, X. Zhang, SupraBank 2024, Host–Guest Complexation of Amphiphilic Molecules at the Air–Water Interface Prevents Oxidation by Hydroxyl Radicals and Singlet Oxygen (dataset). https://doi.org/10.34804/supra.20210928355

Link: https://doi.org/10.34804/supra.20210928355
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

W. C. Geng, D. Zhang, C. Gong, Z. Li, K. M. Barraza, J. L. Beauchamp, D. S. Guo, X. Zhang, Angew. Chem. Int. Ed. 2020, 59, 12684–12688.

Link: https://doi.org/10.1002/anie.202001355
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of oleate (3.2786885245901638e-06 M) and Guanidinocalix[5]arene pentadodecyl ether (0 — 6.5573770491803276e-06 M).