Interaction

curated

Interaction Scheme

Molecule

Fluorescein
Fluorescein

c = 0.5 µM

Host

Gcx5 12c
Guanidinocalix[5]arene pentadodecyl ether

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.90⋅107 ± 5.00⋅106 M-1
Kd =
logKa = 7.28 ± 0.12
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -41.55 ± 0.67 -9.93 ± 0.16
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 500.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM HEPES pH-7.4
Solvents water 100.0 %
Additives Hepes 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

X. Hu, J. Gao, F. Chen, D. Guo, SupraBank 2024, A host-guest drug delivery nanosystem for supramolecular chemotherapy (dataset). https://doi.org/10.34804/supra.2021092866

Link: https://doi.org/10.34804/supra.2021092866
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

X.-Y. Hu, J. Gao, F.-Y. Chen, D.-S. Guo, Journal of Controlled Release 2020, 324, 124–133.

Link: https://doi.org/10.1016/j.jconrel.2020.05.008
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Fluorescein (1.0526315789473683e-06 M) and Guanidinocalix[5]arene pentadodecyl ether (0 — 2.1052631578947366e-06 M).