Interaction

curated

Interaction Scheme

Molecule

Lucigenin
Lucigenin

c = 1.0 µM

Host

Scx4 na
sCx4 sodium salt

c = 0.0 — 6.2 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.60⋅106 ± 1.00⋅105 M-1
Kd =
logKa = 6.2 ± 0.03
T 37.0 °C ≈ 310 K
Energy kJ mol-1 kcal mol-1
ΔG = -36.84 ± 0.16 -8.8 ± 0.04
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 368.0 nm
𝛌em = 510.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM phosphate pH-2.0
Solvents water
Additives Phosphoric acid 10.0 mM
Sodium hydroxide
Source of Concentration estimated
Total concentration 10.0 mM
pH 2.0
Please find here information about the dataset this interaction is part of.
Citation:

D. Guo, Z. Zheng, W. Geng, H. Li, SupraBank 2024, Sensitive fluorescence detection of saliva pepsin by a supramolecular tandem assay enables the diagnosis of gastroesophageal reflux disease (dataset). https://doi.org/10.34804/supra.20210928256

Link: https://doi.org/10.34804/supra.20210928256
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

Z. Zheng, W.-C. Geng, H.-B. Li, D.-S. Guo, Supramolecular Chemistry 2020, 1–8.

Link: https://doi.org/10.1080/10610278.2020.1857762
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Lucigenin (1.25e-05 M) and sCx4 sodium salt (0 — 2.5e-05 M).