Interaction Scheme
Molecule
EY
c = 0.5 µM
Host
gCx4-OEG
c = 0.0 — 13.8 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 2.37⋅105 | ± 1.20⋅104 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -30.68 | ± 0.13 | -7.33 | ± 0.03 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 517.0 nm | ||
| 𝛌em | = | 537.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM HEPES pH-7.4 |
| Solvents | water | 100.0 % |
| Additives | Hepes | 10.0 mM |
| Source of Concentration | ||
| Total concentration | 10.0 mM | |
| pH | 7.4 |
Please find here information about the dataset this interaction is part of.
| Citation: |
D. Guo, R. Wang, Q. Huang, H. Zhao, M. Shui, SupraBank 2024, Heparin reversal by an oligoethylene glycol functionalized guanidinocalixarene (dataset). https://doi.org/10.34804/supra.20210928258 |
| Link: | https://doi.org/10.34804/supra.20210928258 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
Q. Huang, H. Zhao, M. Shui, D.-S. Guo, R. Wang, Chem. Sci. 2020, 11, 9623–9629. |
| Link: | https://doi.org/10.1039/d0sc03922e |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of EY (8.438818565400843e-05 M) and gCx4-OEG (0 — 0.00016877637130801687 M).
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