Interaction

curated

Interaction Scheme

Molecule

Compound
Insuline human

c = 10.0 µM

Host

Scx4 12c
sCx4-12C

Indicator

Lucigenin
Lucigenin

c = 10.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2.00⋅107 ± 5.00⋅106 M-1
Kd =
logKa = 7.3 ± 0.11
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -41.67 ± 0.63 -9.96 ± 0.15
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 420.0 nm
𝛌em = 510.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM phosphate pH-7.4
Solvents water 100.0 %
Additives Disodium phosph... 7.5 mM
Sodium dihydro... 2.5 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

X. Hu, D. Guo, Y. Pan, H. Tian, H. Zhao, X. Yang, SupraBank 2024, Inhibition of insulin fibrillation by amphiphilic sulfonatocalixarene (dataset). https://doi.org/10.34804/supra.20210928362

Link: https://doi.org/10.34804/supra.20210928362
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

H. Zhao, X.-H. Yang, Y.-C. Pan, H.-W. Tian, X.-Y. Hu, D.-S. Guo, Chinese Chemical Letters 2020, 31, 1873–1876.

Link: https://doi.org/10.1016/j.cclet.2020.01.042
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Insuline human (1.0e-06 M) and sCx4-12C (0 — 2.0e-06 M).