Interaction
Interaction Scheme
Molecule
Lucigenin
c = 0.2 µM
Host
sCx5-12C
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 2.70⋅107 | ± 3.00⋅106 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -42.42 | ± 0.28 | -10.14 | ± 0.07 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 368.0 nm | ||
| 𝛌em | = | 507.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 10 mM HEPES pH-7.4 |
| Solvents | water | 100.0 % |
| Additives | Hepes | 10.0 mM |
| Source of Concentration | ||
| Total concentration | 10.0 mM | |
| pH | 7.4 |
Please find here information about the dataset this interaction is part of.
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
H.-W. Tian, Y.-X. Chang, X.-Y. Hu, M. R. Shah, H.-B. Li, D.-S. Guo, Nanoscale 2021, 13, 15362–15368. |
| Link: | https://doi.org/10.1039/D1NR04328E |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of Lucigenin (7.407407407407407e-07 M) and sCx5-12C (0 — 1.4814814814814815e-06 M).
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