Interaction Scheme

Molecule

Lucigenin
Lucigenin

c = 0.2 µM

Host

1
sCx5-12C

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2.70⋅107 ± 3.00⋅106 M-1
Kd =
logKa = 7.43 ± 0.05
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -42.42 ± 0.28 -10.14 ± 0.07
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 368.0 nm
𝛌em = 507.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM HEPES pH-7.4
Solvents water 100.0 %
Additives Hepes 10.0 mM
Source of Concentration
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Please find here information about the scholarly article describing the results derived from that data.
Citation:

H.-W. Tian, Y.-X. Chang, X.-Y. Hu, M. R. Shah, H.-B. Li, D.-S. Guo, Nanoscale 2021, 13, 15362–15368.

Link: https://doi.org/10.1039/D1NR04328E
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Lucigenin (7.407407407407407e-07 M) and sCx5-12C (0 — 1.4814814814814815e-06 M).