Interaction

curated

Interaction Scheme

Molecule

Untitled
IR780

c = 0.0 — 2.1 µM

Host

1
SACx4

c = 0.5 µM

Indicator

Rhodamine b
Rhodamine B

c = 0.3 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2.10⋅106 ± 1.00⋅105 M-1
Kd =
logKa = 6.32 ± 0.02
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -36.09 ± 0.12 -8.63 ± 0.03
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 554.0 nm
𝛌em = 575.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM PBS pH-7.4
Solvents water
Additives sodium chloride 136.99 mM
Disodium hydrog... 6.13 mM
Sodium dihydrog... 5.03 mM
potassium chloride 2.7 mM
Source of Concentration estimated
Total concentration 10.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

D. Guo, F. Huang, Y. Kong, F. Yang, T. Zhang, H. Li, X. Hou, Y. Yue, M. R. Shah, J. Liu, SupraBank 2024, A hypoxia-responsive supramolecular formulation for imaging-guided photothermal therapy (dataset). https://doi.org/10.34804/supra.20211124412

Link: https://doi.org/10.34804/supra.20211124412
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

T.-X. Zhang, X. Hou, Y. Kong, F. Yang, Y.-X. Yue, M. R. Shah, H.-B. Li, F. Huang, J. Liu, D.-S. Guo, Theranostics 2022, 12, 396–409.

Link: https://doi.org/10.7150/thno.67036
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of IR780 (9.523809523809523e-06 M) and SACx4 (0 — 1.9047619047619046e-05 M).