Interaction Scheme
Molecule
spermine
c = 0.0 — 0.4 µM
Host
HPTS
c = 0.25 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 2.10⋅106 | M-1 | |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -36.09 | -8.63 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 350.0 nm | ||
| 𝛌em | = | 430.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 0.8 mM MOPS buffer, pH=7 |
| Solvents | water | |
| Source of Concentration | real | |
| Total concentration | 0.8 mM | |
| pH | 7.0 |
Please find here information about the dataset this interaction is part of.
| Citation: |
Z. Köstereli, K. Severin, SupraBank 2025, Fluorescence sensing of spermine with a frustrated amphiphile (dataset). https://doi.org/10.34804/supra.20220203416 |
| Link: | https://doi.org/10.34804/supra.20220203416 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
Z. Köstereli, K. Severin, Chem. Commun. 2012, 48, 5841. |
| Link: | https://doi.org/10.1039/C2CC32228E |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of spermine (9.523809523809523e-06 M) and HPTS (0 — 1.9047619047619046e-05 M).
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