Interaction Scheme

Molecule

Untitled
L-aspartic acid

c = 0.0 — 600000.0 µM

Host

Bu coumarin aldehyde
Bu,NEt2coumarinCHO

c = 10.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 2.3 M-1
Kd =
logKa = 0.36
T 37.0 °C 310 K
Energy kJ mol-1 kcal mol-1
ΔG = -2.15 -0.51
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 495.0 nm
𝛌em = 513.0 nm
IboundIfree = 40.0
Detailed information about the solvation.
Solvent System Buffer System 50 mM HEPES 100 mM NaCl pH-7.4
Solvents water
Additives sodium chloride 100.0 mM
Hepes 50.0 mM
Source of Concentration real
Total concentration 150.0 mM
pH 7.4
Please find here information about the dataset this interaction is part of.
Citation:

T. E. Glass, E. K. Feuster, SupraBank 2024, Detection of Amines and Unprotected Amino Acids in Aqueous Conditions by Formation of Highly Fluorescent Iminium Ions (dataset). https://doi.org/10.34804/supra.20220628443

Link: https://doi.org/10.34804/supra.20220628443
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

E. K. Feuster, T. E. Glass, J. Am. Chem. Soc. 2003, 125, 16174–16175.

Link: https://doi.org/10.1021/ja036434m
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of L-aspartic acid (8.695652173913045 M) and Bu,NEt2coumarinCHO (0 — 17.39130434782609 M).