Interaction Scheme
Molecule
L-Lys
c = 0.0 — 600000.0 µM
Host
Bu,NEt2coumarinCHO
c = 10.0 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 6.5 | M-1 | |
| Kd = | |||
| logKa = | |||
| T | 37.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -4.83 | -1.15 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Direct Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 495.0 nm | ||
| 𝛌em | = | 513.0 nm | ||
| Ibound⁄Ifree | = | 29.0 | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 50 mM HEPES 100 mM NaCl pH-7.4 |
| Solvents | water | |
| Additives | sodium chloride | 100.0 mM |
| Hepes | 50.0 mM | |
| Source of Concentration | real | |
| Total concentration | 150.0 mM | |
| pH | 7.4 |
Please find here information about the dataset this interaction is part of.
| Citation: |
T. E. Glass, E. K. Feuster, SupraBank 2024, Detection of Amines and Unprotected Amino Acids in Aqueous Conditions by Formation of Highly Fluorescent Iminium Ions (dataset). https://doi.org/10.34804/supra.20220628443 |
| Link: | https://doi.org/10.34804/supra.20220628443 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
E. K. Feuster, T. E. Glass, J. Am. Chem. Soc. 2003, 125, 16174–16175. |
| Link: | https://doi.org/10.1021/ja036434m |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of L-Lys (3.076923076923077 M) and Bu,NEt2coumarinCHO (0 — 6.153846153846154 M).
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