Interaction Scheme
Molecule
(BuBr)2DAP
c = 3.3 µM
Host
sP5A
c = 3.1 µM
Indicator
Spermine (fully protonated form)
c = 0.0 — 8.2 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 2.10⋅109 | ± 4.00⋅108 | M-1 |
| Kd = | |||
| logKa = | |||
| T | 25.0 °C | ||
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -53.21 | ± 0.48 | -12.72 | ± 0.11 |
These are the specifications of the determination of the experimental results.
| Detection Method: | Competitive | |||
| Assay Type: | Competitive Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 335.0 nm | ||
| 𝛌em | = | 423.0 nm | ||
Detailed information about the solvation.
| Solvent System | Buffer System | 1X PBS pH-7.0 |
| Solvents | water | |
| Additives | sodium chloride | 137.0 mM |
| Disodium hydrog... | 10.0 mM | |
| potassium chloride | 2.7 mM | |
| Potassium dihyd... | 1.8 mM | |
| Source of Concentration | ||
| Total concentration | 151.5 mM | |
| pH | 7.0 |
Please find here information about the dataset this interaction is part of.
| Citation: |
L. Grimm, SupraBank 2024, Pillar[n]arene-based fluorescence turn-on chemosensors for the detection of spermine, spermidine, and cadaverine in saline media and biofluids (dataset). https://doi.org/10.34804/supra.20240313515 |
| Link: | https://doi.org/10.34804/supra.20240313515 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
|
| Link: | https://suprabank.org/datasets/515 |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of (BuBr)2DAP (9.523809523809524e-09 M) and sP5A (0 — 1.9047619047619048e-08 M).
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