Interaction

uncurated

Interaction Scheme

Molecule

Memb7
MemB7

c = 0.0 — 100.0 µM

Host

Cb8
CB8

c = 1.3 µM

Indicator

Methylene blue
Methylene Blue

c = 2.6 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.40⋅109 ± 2.00⋅108 M-1
Kd =
logKa = 9.15 ± 0.06
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -52.21 ± 0.36 -12.48 ± 0.09
Comment
Binding Properties where determined by ITC this assay confirmed Ka
These are the specifications of the determination of the experimental results.
Detection Method: Competitive
Assay Type: Competitive Binding Assay
Technique: Fluorescence
𝛌ex = 611.0 nm
𝛌em = 685.0 nm
Detailed information about the solvation.
Solvent System Single Solvent
Solvent water
Please find here information about the dataset this interaction is part of.
Citation:

D. Fuentealba, D. Guerra Díaz, N. Mariño-Ocampo, D. Zúñiga-Núñez, M. Soto-Arriaza, D. González-Torres, M. Canals Díaz, C. Muñoz, SupraBank 2025, Memantine-biotin conjugate with intra tumoral cell displacing ability for cucurbit[8]uril-based switchable photosensitizer (dataset). https://doi.org/10.34804/supra.20250408595

Link: https://doi.org/10.34804/supra.20250408595
Export: BibTex | RIS | EndNote
Please find here information about the scholarly article describing the results derived from that data.
Citation:

D. González-Torres, D. Zúñiga-Núñez, N. Mariño-Ocampo, M. Canals Díaz, C. Muñoz, D. Guerra Díaz, M. Soto-Arriaza, D. Fuentealba, Journal of Photochemistry and Photobiology A: Chemistry 2025, 463, 116305.

Link: https://doi.org/10.1016/j.jphotochem.2025.116305
Export: BibTex | RIS | EndNote

Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of MemB7 (1.4285714285714286e-08 M) and CB8 (0 — 2.8571428571428572e-08 M).