Dataset curated
- DOI:
- https://doi.org/10.34804/supra.20210928246
- Title:
- Interfacial Recognition of Acetylcholine by an Amphiphilic p-Sulfonatocalix[8]arene Derivative Incorporated into Dimyristoyl Phosphatidylcholine Vesicles
- Description:
- Dodecyl ether derivatives 1-3 of p-sulfonatocalix[n]arene were incorporated into dimyristoyl phosphatidylcholine (DMPC) vesicles, and their binding abilities for acetylcholine (ACh) were examined by using steady-state fluorescence/fluorescence anisotropy and fluorescence correlation spectroscopy (FCS). For the detection of ACh binding to the DMPC vesicles containing 5 mol % of 1-3, competitive fluorophore displacement experiments were performed, where rhodamine 6G (Rh6G) was used as a fluorescent guest. The addition of Rh6G to the DMPC vesicles containing 3 resulted in a decrease in the fluorescence intensity of Rh6G with an increase of its fluorescence anisotropy, indicating that Rh6G binds to the DMPC-3 vesicles. In the case of DMPC-1 and DMPC-2 vesicles, significant changes in the fluorescence spectra of Rh6G were not observed. When ACh was added to the DMPC-3 vesicles in the presence of Rh6G ([3]/[Rh6G]=100), the fluorescence intensity of Rh6G increased with a decrease in its fluorescence anisotropy. From the analysis of fluorescence titration data, the association constants were determined to be 7.1×105 M-1 for Rh6G-3 complex and 1.1×102 M-1 for ACh-3 complex at the DMPC-3 vesicles. To get a direct evidence for the binding of Rh6G and its displacement by ACh at the DMPC-3 vesicles, diffusion times of the Rh6G were measured by using FCS. Binding selectivity of the DMPC-3 vesicles for ACh, choline, GABA, L-aspartic acid, L-glutamic acid, L-arginine, L-lysine, L-histamine and ammonium chloride was also evaluated using FCS.
| Citation: |
T. Jin, F. Fujii, Y. Ooi, SupraBank 2024, Interfacial Recognition of Acetylcholine by an Amphiphilic p-Sulfonatocalix[8]arene Derivative Incorporated into Dimyristoyl Phosphatidylcholine Vesicles (dataset). https://doi.org/10.34804/supra.20210928246 |
| Link: | https://doi.org/10.34804/supra.20210928246 |
| Export: | BibTex | RIS | EndNote |
| Views | Downloads | Citations |
|---|---|---|
| 886 | 0 | 0 |
| Citation: |
T. Jin, F. Fujii, Y. Ooi, Sensors 2008, 8, 6777–6790. |
| Link: | https://doi.org/10.3390/s8106777%20 |
| Export: | BibTex | RIS | EndNote |
- Creators:
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Takashi Jin
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Fumihiko Fujii
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Yasuhiro Ooi
- Contributers:
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Anxhela Zhiti
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DataManager
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