Dataset curated
- DOI:
- https://doi.org/10.34804/supra.20220628439
- Title:
- Eukaryotic Cell Toxicity and HSA Binding of [Ru(Me4phen)(bb7)]2+ and the Effect of Encapsulation in Cucurbit[10]uril
- Description:
- The toxicity (IC50) of a series of mononuclear ruthenium complexes containing bis[4(4′-methyl-2,2′-bipyridyl)]-1,n-alkane (bbn) as a tetradentate ligand against three eukaryotic cell lines—BHK (baby hamster kidney), Caco-2 (heterogeneous human epithelial colorectal adenocarcinoma) and Hep-G2 (liver carcinoma)—have been determined. The results demonstrate that cis-α-[Ru(Me4phen)(bb7)]2+ (designated as α-Me4phen-bb7, where Me4phen = 3,4,7,8-tetramethyl-1,10-phenanthroline) showed little toxicity toward the three cell lines, and was considerably less toxic than cis-α-[Ru(phen)(bb12)]2+ (α-phen-bb12) and the dinuclear complex [{Ru(phen)2}2{μ-bb12}]4+. Fluorescence spectroscopy was used to study the binding of the ruthenium complexes with human serum albumin (HSA). The binding of α-Me4phen-bb7 to the macrocyclic host molecule cucurbit[10]uril (Q[10]) was examined by NMR spectroscopy. Large upfield 1H NMR chemical shift changes observed for the methylene protons in the bb7 ligand upon addition of Q[10], coupled with the observation of several intermolecular ROEs in ROESY spectra, indicated that α-Me4phen-bb7 bound Q[10] with the bb7 methylene carbons within the cavity and the metal center positioned outside one of the portals. Simple molecular modeling confirmed the feasibility of the binding model. An α-Me4phen-bb7-Q[10] binding constant of 9.9 ± 0.2 × 106 M−1 was determined by luminescence spectroscopy. Q[10]-encapsulation decreased the toxicity of α-Me4phen-bb7 against the th...
| Citation: |
A. I. Day, B. Sun, I. F. Musgrave, K. Heimann, F. R. Keene, J. G. Collins, SupraBank 2024, Eukaryotic Cell Toxicity and HSA Binding of [Ru(Me4phen)(bb7)]2+ and the Effect of Encapsulation in Cucurbit[10]uril (dataset). https://doi.org/10.34804/supra.20220628439 |
| Link: | https://doi.org/10.34804/supra.20220628439 |
| Export: | BibTex | RIS | EndNote |
| Views | Downloads | Citations |
|---|---|---|
| 854 | 0 | 0 |
| Citation: |
B. Sun, I. F. Musgrave, A. I. Day, K. Heimann, F. R. Keene, J. G. Collins, Front. Chem. 2018, 6, DOI 10.3389/fchem.2018.00595. |
| Link: | https://doi.org/10.3389/fchem.2018.00595 |
| Export: | BibTex | RIS | EndNote |
- Creators:
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Biyun Sun
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Ian F. Musgrave
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Anthony I. Day
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Kirsten Heimann
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F. Richard Keene
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J. Grant Collins
- Contributers:
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Nilima Manoj Kumar
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DataManager
- Subjects:
- general chemistry
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