Interaction

curated

Interaction Scheme

Molecule

Lucigenin
Lucigenin

c = 1.0 µM

Host

Scx4
sCx4

c = 0.0 — 25.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 7.00⋅104 ± 1.00⋅104 M-1
Kd =
logKa = 4.85 ± 0.06
T 25.0 °C ≈ 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -27.66 ± 0.36 -6.61 ± 0.09
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 460.0 nm
𝛌em = 505.0 nm
Detailed information about the solvation.
Solvent System Complex Mixture
Solvents water 100.0 %
water
Additives Sodium perchlorate 100.0 mM
Total concentration 100.0 mM
pH 7.0
Please find here information about the dataset this interaction is part of.
Citation:

D. Guo, Y. Liu, W. M. Nau, V. D. Uzunova, X. Su, SupraBank 2024, Operational calixarene-based fluorescent sensing systems for choline and acetylcholine and their application to enzymatic reactions (dataset). https://doi.org/10.34804/supra.2021092878

Link: https://doi.org/10.34804/supra.2021092878
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

D.-S. Guo, V. D. Uzunova, X. Su, Y. Liu, W. M. Nau, Chem. Sci. 2011, 2, 1722.

Link: https://doi.org/10.1039/C1SC00231G%20
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Binding Isotherm Simulations

The plot depicts the binding isotherm simulation of a 1:1 interaction of Lucigenin (0.00028571428571428574 M) and sCx4 (0 — 0.0005714285714285715 M).