Interaction Scheme

Molecule

Untitled
Rhodamine 6G

c = 0.02 µM

Host

Scx8
sCx8

c = 0.0 — 20.0 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.90⋅106 M-1
Kd =
logKa = 6.28
T 25.0 °C 298 K
kin= ± M-1s-1
kout= 0.0 ± s-1
Energy kJ mol-1 kcal mol-1
ΔG = -35.84 -8.57
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 480.0 nm
𝛌em = 550.0 nm
Detailed information about the solvation.
Solvent System Buffer System 10 mM phosphate pH-6.9
Solvents water 100.0 %
Additives Disodium phosph... 5.3 mM
Sodium dihydro... 4.7 mM
Source of Concentration
Total concentration 10.0 mM
pH 6.9
Please find here information about the dataset this interaction is part of.
Citation:

T. Jin, F. Fujii, Y. Ooi, SupraBank 2024, Interfacial Recognition of Acetylcholine by an Amphiphilic p-Sulfonatocalix[8]arene Derivative Incorporated into Dimyristoyl Phosphatidylcholine Vesicles (dataset). https://doi.org/10.34804/supra.20210928246

Link: https://doi.org/10.34804/supra.20210928246
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Please find here information about the scholarly article describing the results derived from that data.
Citation:

T. Jin, F. Fujii, Y. Ooi, Sensors 2008, 8, 6777–6790.

Link: https://doi.org/10.3390/s8106777%20
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Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Rhodamine 6G (1.0526315789473684e-05 M) and sCx8 (0 — 2.105263157894737e-05 M).