Interaction Scheme
Molecule
Berberine chloride
c = 0.6 µM
Host
CB8
c = 0.0 — 0.31 µM
Cofactor
Berberine chloride
c = 0.6 µM
Binding Properties
| 𝜈 | Molecule 1 : 1 Host | ||
| Ka = | 8.25⋅106 | M-1 | |
| Kd = | |||
| logKa = | |||
| T | 10.0 °C | ||
| kin= | 5000000.0 | ± 500000.0 | M-1s-1 |
| kout= | 0.606060606060606 | ± 0.1 | s-1 |
| Energy | kJ mol-1 | kcal mol-1 | |||
|---|---|---|---|---|---|
| ΔG | = | -37.49 | ± NaN | -8.96 | ± NaN |
These are the specifications of the determination of the experimental results.
| Detection Method: | Direct | |||
| Assay Type: | Associative Binding Assay | |||
| Technique: | Fluorescence | |||
| 𝛌ex | = | 345.0 nm | ||
| 𝛌em | = | 535.0 nm | ||
Detailed information about the solvation.
| Solvent System | Single Solvent |
| Solvent | water |
| pH | 7.0 |
Please find here information about the dataset this interaction is part of.
| Citation: |
Z. Miskolczy, L. Biczok, SupraBank 2024, Sequential inclusion of two berberine cations in cucurbit[8]uril cavity: kinetic and thermodynamic studies (dataset). https://doi.org/10.34804/supra.202109285 |
| Link: | https://doi.org/10.34804/supra.202109285 |
| Export: | BibTex | RIS | EndNote |
Please find here information about the scholarly article describing the results derived from that data.
| Citation: |
Z. Miskolczy, L. Biczók, Phys. Chem. Chem. Phys. 2014, 16, 20147–20156. |
| Link: | https://doi.org/10.1039/C4CP02919D |
| Export: | BibTex | RIS | EndNote | |
Binding Isotherm Simulations
The plot depicts the binding isotherm simulation of a 1:1 interaction of Berberine chloride (2.4242424242424244e-06 M) and CB8 (0 — 4.848484848484849e-06 M).
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