Interaction Scheme

Molecule

Bc
Berberine chloride

c = 0.8 µM

Host

Cb7
CB7

c = 0.0 — 2.7 µM

Binding Properties

𝜈 Molecule 1 : 1 Host
Ka = 1.70⋅107 ± 3.94⋅106 M-1
Kd =
logKa = 7.23 ± 0.1
T 25.0 °C 298 K
Energy kJ mol-1 kcal mol-1
ΔG = -41.27 ± 0.59 -9.86 ± 0.14
These are the specifications of the determination of the experimental results.
Detection Method: Direct
Assay Type: Direct Binding Assay
Technique: Fluorescence
𝛌ex = 421.0 nm
𝛌em = 542.0 nm
Detailed information about the solvation.
Solvent System Single Solvent
Solvent water
Please find here information about the dataset this interaction is part of.
Citation:

F. Biedermann, S. Sinn, J. Krämer, SupraBank 2022, Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA) (dataset). https://doi.org/10.34804/supra.20210928361

Link: https://doi.org/10.34804/supra.20210928361
Export: BibTex | RIS | EndNote
Please find here information about the scholarly article describing the results derived from that data.
Citation:

S. Sinn, J. Krämer, F. Biedermann, Chem. Commun. 2020, 56, 6620–6623.

Link: https://doi.org/10.1039/D0CC01841D
Export: BibTex | RIS | EndNote |

Binding Isotherm Simulations


The plot depicts the binding isotherm simulation of a 1:1 interaction of Berberine chloride (1.1776873110313855e-06 M) and CB7 (0 — 2.355374622062771e-06 M).